Basic Information

IDDRAMP21352
SequenceIHHIHHIIHHIHHI
Length14
Name3IH2 (De Novo Synthesis)
Sourcesynthetic construct
ActivityAntimicrobial, Antibacterial, Anti-Gram-
Pathogen[Ref.31199117] Gram-negative Bacteria: Escherichia coli 25922 (MIC=4μM), Escherichia coli UB1005 (MIC=8μM), Escherichia coli K88 (MIC=4μM), Escherichia coli K99 (MIC=4μM), Escherichia coli 078 (MIC=8μM), Escherichia coli 987P (MIC=2μM), Pseudomonas aeruginosa 27853 (MIC=4μM), Pseudomonas aeruginosa PAO1 (MIC=4μM), Salmonella typhimurium 14028 (MIC=32μM), Salmonella typhimurium 7731 (MIC=16μM)
Hemolytic Activity[Ref.31199117] 5% hemolysis at 63μM, 8% hemolysis at 128μM against human red blood cells
Cytotoxicity[Ref.31199117] ①The cell viability of HEK293T cells induced by 3IH2 is 105.8%, 105.8%, 105.2%, 104.2%, 101.1%, 101.7% and 100.8% at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM at pH 7.4, while that induced by Melittin is 47.2%, 1.7%, 1.9%, 0.6%, 0.6%, 0.6% and 1.7% at 2, 4, 8, 16, 32, 64 and 128 μM. ②The cell viability of HEK293T cells induced by 3IH2 is 106.2%, 105.9%, 105.9%, 105.6%, 104.2%, 104.8% and 101.4% at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM at pH 6.0, while that induced by Melittin is 43.8%, 3.4%, 3.4%, 0%, 2.2%, 0% and 1.1% at 2, 4, 8, 16, 32, 64 and 128 μM.
N-terminal ModificationFree
C-terminal ModificationAmidation
Linear/Cyclic/BranchedLinear
Uniprot
PDB
3D View
PDB DownloadDownload PDB Predicted by Alphafold2
PubMed ID31199117

Physicochemical Properties

Residues14
SequenceIHHIHHIIHHIHHI
Molecular Weight1794.075
Grand Average of Hydropathy0.1
Isoelectric Point7.284
Charge at pH 7.4-0.15
Secondary StructureHelix: 0.429, Turn: 0.000, Sheet: 0.000
Instability Index170.086
Aromaticity0

Similar Structure Search

Candidates based on structure

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