Basic Information

IDDRAMP21350
SequenceIHHIHHHIHHIHHHIHHIHHHIHHIHHH
Length28
Name2IH4 (De Novo Synthesis)
Sourcesynthetic construct
ActivityAntimicrobial, Antibacterial, Anti-Gram-
Pathogen[Ref.31199117] Gram-negative Bacteria: Escherichia coli 25922 (MIC=1μM), Escherichia coli UB1005 (MIC=2μM), Escherichia coli K88 (MIC=4>64μM), Escherichia coli K99 (MIC=1μM), Escherichia coli 078 (MIC=4>64μM), Escherichia coli 987P (MIC=1μM), Pseudomonas aeruginosa 27853 (MIC=1μM), Pseudomonas aeruginosa PAO1 (MIC=1μM), Salmonella typhimurium 14028 (MIC=64μM), Salmonella typhimurium 7731 (MIC>64μM)
Hemolytic Activity[Ref.31199117] 5% hemolysis at 63μM, 8% hemolysis at 128μM against human red blood cells
Cytotoxicity[Ref.31199117] ①The cell viability of HEK293T cells induced by 2IH4 is at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM at pH 7.4, while that induced by Melittin is 47.2%, 1.7%, 1.9%, 0.6%, 0.6%, 0.6% and 1.7% at 2, 4, 8, 16, 32, 64 and 128 μM. ②The cell viability of HEK293T cells induced by 2IH4 is 103.7%, 103.7%, 104.8%, 103.4%, 105.3%, 94.9% and 57.9% at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM at pH 6.0, while that induced by Melittin is 43.8%, 3.4%, 3.4%, 0%, 2.2%, 0% and 1.1% at 2, 4, 8, 16, 32, 64 and 128 μM.
N-terminal ModificationFree
C-terminal ModificationAmidation
Linear/Cyclic/BranchedLinear
Uniprot
PDB
3D View
PDB DownloadDownload PDB Predicted by Alphafold2
PubMed ID31199117

Physicochemical Properties

Residues28
SequenceIHHIHHHIHHIHHHIHHIHHHIHHIHHH
Molecular Weight3666.062
Grand Average of Hydropathy-1
Isoelectric Point7.547
Charge at pH 7.40.29
Secondary StructureHelix: 0.286, Turn: 0.000, Sheet: 0.000
Instability Index154.75
Aromaticity0

Similar Structure Search

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