Basic Information

IDDRAMP21354
SequenceIHHIHHIIHHIHHIIHHIHHIIHHIHHI
Length28
Name3IH4 (De Novo Synthesis)
Sourcesynthetic construct
ActivityAntimicrobial, Antibacterial, Anti-Gram-
Pathogen[Ref.31199117] Gram-negative Bacteria: Escherichia coli 25922 (MIC=2μM), Escherichia coli UB1005 (MIC=4μM), Escherichia coli K88 (MIC=2μM), Escherichia coli K99 (MIC=4μM), Escherichia coli 078 (MIC=4μM), Escherichia coli 987P (MIC=4μM), Pseudomonas aeruginosa 27853 (MIC=8μM), Pseudomonas aeruginosa PAO1 (MIC=4μM), Salmonella typhimurium 14028 (MIC>64μM), Salmonella typhimurium 7731 (MIC>64μM)
Hemolytic Activity[Ref.31199117] 8% hemolysis at 63μM, 15% hemolysis at 128μM against human red blood cells
Cytotoxicity[Ref.31199117] ①The cell viability of HEK293T cells induced by 3IH4 is 106.9%, 101.1%, 101.9%, 103.6%, 102.5%, 101.7% and 80.6% at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM at pH 7.4, while that induced by Melittin is 47.2%, 1.7%, 1.9%, 0.6%, 0.6%, 0.6% and 1.7% at 2, 4, 8, 16, 32, 64 and 128 μM. ②The cell viability of HEK293T cells induced by 3IH4 is 104.2%, 105.3, 104.2%, 104.2%, 101.4%, 100.6%, 56.5% at peptide concentrations of 2, 4, 8, 16, 32, 64 and 128 μM at pH 6.0, while that induced by Melittin is 43.8%, 3.4%, 3.4%, 0%, 2.2%, 0% and 1.1% at 2, 4, 8, 16, 32, 64 and 128 μM.
N-terminal ModificationFree
C-terminal ModificationAmidation
Linear/Cyclic/BranchedLinear
Uniprot
PDB
3D View
PDB DownloadDownload PDB Predicted by Alphafold2
PubMed ID31199117

Physicochemical Properties

Residues28
SequenceIHHIHHIIHHIHHIIHHIHHIIHHIHHI
Molecular Weight3570.135
Grand Average of Hydropathy0.1
Isoelectric Point7.481
Charge at pH 7.40.143
Secondary StructureHelix: 0.429, Turn: 0.000, Sheet: 0.000
Instability Index170.443
Aromaticity0

Similar Structure Search

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