| Cytotoxicity | [Ref.31242693] The IC50 values which represents the inhibitory effect of Ranatuerin-2Pb on the proliferation of NCI-H157, MCF-7, U251MG and PC-3 are 1.453 μM, 7.254 μM, 2.172 μM and 2.251 μM. In addtion, Ranatuerin-2Pb showed no inhibitory effect on the proliferation of MDA-MB-435s. DETAILED DATA: ①The cell viabillity of NCI-H157 induced by Ranatuerin-2Pb is 96.2%, 96.9%, 85.6%, 60%, 5% and 4.4% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 100%, 95%, 89.4%, 41.2%, 16.2% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ②The cell viability of MCF-7 induced by Ranatuerin-2Pb is 100.6%, 92.5%, 90%, 99.4%, 36.2% and 11.2% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 100%, 98.1%, 92.5%, 7.5%, 8.7% and 8.7% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ③The cell viability of U251-MG induced by Ranatuerin-2Pb is 100%, 105.6%, 108.1%, 102.5%, 59.4% and 17.5% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 99.4%, 99.4%, 75.6%, 18.7%, 11.9% and 18.1% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ④The cell viability of PC-3 induced by Ranatuerin-2Pb is 108.7%, 112.5%, 84.4%, 80.6%, 28.1% and 16.2% at peptide concentrations of 0.001, 0.01, 0.1, 1, 10, 100 μM, while the cell viability induced by Melittin is 110%, 113.1%, 109.4%, 33.1%, 16.9% and 16.2% at 0.001, 0.01, 0.1, 1, 10, 100 μM. ⑤ The cell viability of MDA-MB-435S induced by Ranatuerin-2Pb is 96.9%, 88.8%, 96.9%, 88.1%, 100 and 25.6%, while the cell viability induced by Melittin is 100%, 93.1%, 82.5%, 18.8%, 18.8% and 18.1%. |
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