| ID | DRAMP21337 |
|---|---|
| Sequence | GRFKRFRKKFKKLFKKLSPVIPLLHL |
| Length | 26 |
| Name | BMAP-27 (Bovine, mammals, animals) |
| Source | Bos taurus (Bovine) |
| Activity | Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- |
| Pathogen | [Ref.31226350] Gram-positive bacteria:Staphylococcus aureus (MIC=1-2 μM);##Gram-negative bacteria: Escherichia coli (MIC=2-4 μM) |
| Hemolytic Activity | [Ref.31226350] 50% hemolysis at 75 μM for human red blood cells |
| Cytotoxicity | [Ref.31226350] The CC50 values which represent peptide concentration (μM) inducing 50% cytotoxicity to human cancer cell lines of BMAP-27 on MDA361 and A549 are 4.2 μM and 5.3 μM. DETAILED DATA: ①The cell viability of MDA-361 induced by BMAP-27 is 100%, 100%, 98.9%, 97.7%, 87.6%, 59.8%, 34.7%, 12.4%, 10.3%, 8.3% and 9.4% at peptide concentrations of 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, 13, 25, 50 and 100 μM. ②The cell viability of A549 induced by BMAP-27 is 100%, 99.1%, 97.7%, 94.7%, 74.5%, 41.4%, 24.4%, 11.3%, 9.7%, 10.3% and 7.4% at peptide concentrations of 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, 13, 25, 50 and 100 μM. |
| N-terminal Modification | Free |
| C-terminal Modification | Amidation |
| Linear/Cyclic/Branched | Linear |
| Uniprot | P54228 |
| PDB | 2KET resolved by NMR |
| 3D View | |
| PDB Download | Download PDB Predicted by Alphafold2 |
| PubMed ID | 31226350 |
Physicochemical Properties
| Residues | 26 |
|---|---|
| Sequence | GRFKRFRKKFKKLFKKLSPVIPLLHL |
| Molecular Weight | 3226.049 |
| Grand Average of Hydropathy | -0.365 |
| Isoelectric Point | 12 |
| Charge at pH 7.4 | 9.576 |
| Secondary Structure | Helix: 0.423, Turn: 0.154, Sheet: 0.192 |
| Instability Index | 21.481 |
| Aromaticity | 0.154 |