| ID | DRAMP29048 |
|---|---|
| Sequence | KIAKRIWKILRRR |
| Length | 13 |
| Name | PA-13 |
| Source | Synthetic construct |
| Activity | Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- |
| Pathogen | [Ref.32499514] Gram-negative bacteria: Pseudomonas aeruginosa ATCC 27853 (MIC = 3.91 µg/ml), Escherichia coli ATCC 25922 (MIC = 31.25 µg/ml), Escherichia coli O157:H7 MT strain (MIC = 31.25 µg/ml), Shigella sonnei ATCC 11060 (MIC = 3.91 µg/ml), Salmonella Typhimurium ATCC 13311 (MIC = 1.95 µg/ml), Vibrio cholera O1 Inaba DMST 16261 (MIC = 31.25 µg/ml), Acinetobacter baumannii MT strain (MIC = 7.81 µg/ml). ##Clinically isolated MDR P. aeruginosa: P. aeruginosa No.1 (3.91 μg/mL), P. aeruginosa No.2 (7.81μg/mL), P. aeruginosa No.3 (7.81 μg/mL), P. aeruginosa No.4 (7.81 μg/mL), P. aeruginosa No.5 (7.81 μg/mL), P. aeruginosa No.6 (7.81 μg/mL), P. aeruginosa No.7 (7.81 μg/mL), P. aeruginosa No.8 (7.81 μg/mL), P. aeruginosa No.9 (7.81 μg/mL), P. aeruginosa No.10 (7.81 μg/mL), P. aeruginosa No.11 (7.81 μg/mL), P. aeruginosa No.12 (3.91 μg/mL), P. aeruginosa No.13 (15.63 μg/mL), P. aeruginosa No.14 (7.81 μg/mL),##Gram-positive bacteria:Staphylococcus aureus ATCC 25923 (MIC = 62.5 µg/ml) Staphylococcus epidermidis ATCC 12228 (MIC = 3.91 µg/ml), Bacillus cereus ATCC 11778 (MIC = 15.63 µg/ml), Enterococcus faecalis ATCC 29212 (MIC = 250 µg/ml), Listeria monocytogenes 10403 s (MIC = 3.91 µg/ml) |
| Hemolytic Activity | [Ref.32499514]MHC = 125 μg/mL. Note:MHC is the minimum concentration that caused 10% hemolysis of human red blood cells (hRBC). When no 10% hemolysis was observed at 250 µg/ml, a value of 500 µg/ml was used to calculate the therapeutic index. |
| Cytotoxicity | [Ref.32499514]Cytotoxic activity of PA-13 against the L929 mouse fibroblast cell line was determined by a colorimetric MTT viability assay. At the MIC (3.91 µg/ml), the cell survival was up to 90%. Even at the maximum concentration (250 µg/ml), the survival was common (81.29%). Melittin, used as a positive control, showed a strong and dose-dependent cytotoxic effect; no living cells were detected at 62.5 µg/ml. Peptide PA-13 displayed very little cytotoxicity on L929 cells when compared to melittin. |
| N-terminal Modification | Free |
| C-terminal Modification | Amidation |
| Linear/Cyclic/Branched | Linear |
| Uniprot | |
| PDB | |
| 3D View | |
| PDB Download | Download PDB Predicted by Alphafold2 |
| PubMed ID | 32499514 |
Physicochemical Properties
| Residues | 13 |
|---|---|
| Sequence | KIAKRIWKILRRR |
| Molecular Weight | 1737.193 |
| Grand Average of Hydropathy | -0.885 |
| Isoelectric Point | 12 |
| Charge at pH 7.4 | 6.55 |
| Secondary Structure | Helix: 0.385, Turn: 0.000, Sheet: 0.154 |
| Instability Index | 139 |
| Aromaticity | 0.077 |