Basic Information
| ID | DRAMP29050 |
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| Sequence | GLLKRIKALL |
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| Length | 10 |
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| Name | Ano-8β (variant of Anoplin) |
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| Source | Synthetic construct (Ano-1β is a variant of Anoplin, which is a linear cationic α-helical AMPs isolated from the venom sac of anoplius samariensis(solitary spider wasps)) |
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| Activity | Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram- |
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| Pathogen | [Ref.32654770] Gram-negative multidrug-resistant bacteria: P. aeruginosa 119 (MIC = 8 μM or 9.00 μg/mL), P. aeruginosa 124 (MIC = 16 μM or 17.98 μg/mL), P. aeruginosa 86 (MIC = 8 μM or 9.00 μg/mL); ##Gram-negative bacteria: P. aeruginosa ATCC 27853 (MIC = 4 μM or 4.50 μg/mL), P. aeruginosa ATCC 9027 (MIC = 8 μM or 9.00 μg/mL), E. coli ATCC 25922 (MIC = 32 μM or 36 μg/mL), K. pneumonia ATCC 700603 (MIC = 128 μM or 144 μg/mL), A. baumannii ATCC 19606 (MIC > 128 μM or 144 μg/mL); ##Gram-positive bacteria: S. aureus ATCC 25923 (MIC = 128 μM or 144 μg/mL), S. epidermidis ATCC 12228 (MIC = 16 μM or 18 μg/mL) |
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| Hemolytic Activity | [Ref.32654770]Ano-8β induced inappreciable hemolytic activity with less than 5 % hemolysis at all the tested concentrations and HC > 256 μM. Note: Minimum hemolysis concentration (HC, μM) was defined as the minimal peptide concentration induced 10 % hemolysis. |
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| Cytotoxicity | [Ref.32654770]After 1 h of incubation, Ano-8β, similar to their parent peptide anoplin, maintained more than 80 % cell viability of the two cells at all tested concentrations. After incubating for 24 h, Ano-8β still maintained more than 80 % cell viability of HEK 293 T cells at all tested concentrations but displayed cytotoxicity against Hela cells at the highest concentration (256 μM). The cell viability of Hela after 24 h of incubation is around 70% |
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| N-terminal Modification | Free |
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| C-terminal Modification | Amidation |
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| Linear/Cyclic/Branched | Linear |
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| Uniprot |
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| PDB |
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| 3D View | |
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| PDB Download | Download PDB Predicted by Alphafold2 |
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| PubMed ID | 32654770 |
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