Basic Information

IDDRAMP21417
SequenceWIKAAAKAAAKIW
Length13
NameWI (De Novo Synthesis)
Sourcesynthetic construct
ActivityAntimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
Pathogen[Ref.30897850] Gram-positive bacteria:Staphylococcus aureus ATCC 27853(MIC=32μM), Staphylococcus epidermidis ATCC 12228(MIC=32μM), Staphylococcus faecalis ATCC 29212(MIC=32μM), Bacillus subtilis CMCC 63501(MIC=8μM);##Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC=8μM), Escherichia coli UB 1005 (MIC=8μM), Pseudomonas aeruginosa ATCC 27853(MIC=32μM), Salmonella typhimurium ATCC 14028(MIC=32μM)
Hemolytic Activity[Ref.30897850] 15% hemolysis at 256μM against human red blood cells
Cytotoxicity[Ref.30897850] ①The cell viability of RAW 264.7 induced by WI is 104.0%, 98.2%, 98.6%, 106.5%, 103.6%, 101.8% and 100% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on RAW 264.7 cells (Control samples) is 93.1%, 67.8%, 37.7%, 17.8%, 0%, 1.4% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.②The cell viability of HEK293T induced by WV is 98.9%, 99.3%, 107.2%, 99.6%, 110.5%, 108.3%, 96.7% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on HEK293T cells (Control samples) is 101.4%, 80.1%, 31.9%, 1.4%, 1.4%, 1.8% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.
N-terminal ModificationFree
C-terminal ModificationAmidaton
Linear/Cyclic/BranchedLinear
Uniprot
PDB
3D View
PDB DownloadDownload PDB Predicted by Alphafold2
PubMed ID30897850

Physicochemical Properties

Residues13
SequenceWIKAAAKAAAKIW
Molecular Weight1427.735
Grand Average of Hydropathy0.485
Isoelectric Point10.302
Charge at pH 7.42.55
Secondary StructureHelix: 0.308, Turn: 0.000, Sheet: 0.462
Instability Index-3.831
Aromaticity0.154

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