Basic Information

IDDRAMP21419
SequenceWWKAAAKAAAKWW
Length13
NameWW (De Novo Synthesis)
Sourcesynthetic construct
ActivityAntimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
Pathogen[Ref.30897850] Gram-positive bacteria:Staphylococcus aureus ATCC 27853(MIC=4μM), Staphylococcus epidermidis ATCC 12228(MIC=4μM), Staphylococcus faecalis ATCC 29212(MIC=4μM), Bacillus subtilis CMCC 63501(MIC=4μM);##Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC=2μM), Escherichia coli UB 1005 (MIC=4μM), Pseudomonas aeruginosa ATCC 27853(MIC=8μM), Salmonella typhimurium ATCC 14028(MIC=8μM)
Hemolytic Activity[Ref.30897850] 24% hemolysis at 256μM against human red blood cells
Cytotoxicity[Ref.30897850] ①The cell viability of RAW 264.7 induced by WW is 104.3%, 103.6%, 109.4%, 103.6%, 97.5%, 89.9%, 63.4% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on RAW 264.7 cells (Control samples) is 93.1%, 67.8%, 37.7%, 17.8%, 0%, 1.4% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.②The cell viability of HEK293T induced by WV is 102.9%, 111.6%, 105.1%, 104.3%, 101.8%, 84.8%, 43.5% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM, while the cytotoxicity of WV on HEK293T cells (Control samples) is 101.4%, 80.1%, 31.9%, 1.4%, 1.4%, 1.8% and 1.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 μM.
N-terminal ModificationFree
C-terminal ModificationAmidaton
Linear/Cyclic/BranchedLinear
Uniprot
PDB
3D View
PDB DownloadDownload PDB Predicted by Alphafold2
PubMed ID30897850

Physicochemical Properties

Residues13
SequenceWWKAAAKAAAKWW
Molecular Weight1573.839
Grand Average of Hydropathy-0.346
Isoelectric Point10.302
Charge at pH 7.42.55
Secondary StructureHelix: 0.308, Turn: 0.000, Sheet: 0.462
Instability Index9.231
Aromaticity0.308

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